解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::SOCS3 is essential for regulating the extent, duration, and specificity of cellular responses to cytokines such as G-CSF and IL-6. Here we describe the solution structure of SOCS3, the first structure determined for any SOCS protein, in complex with a phosphotyrosine-containing peptide from the IL-6 receptor signaling...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2006.03.024
更新日期:2006-04-21 00:00:00
abstract::The oncogenic process often leads to a loss of normal telomere length control, usually as a result of activation of telomerase. Nevertheless, there are also telomerase-independent events that involve a Rad50-dependent recombination mechanism to maintain telomere length. Previous work has implicated the Rb family of pr...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2006.02.016
更新日期:2006-04-07 00:00:00
abstract::In this issue of Molecular Cell, detail molecular events that trigger apoptosis following growth factor withdrawal, linking irreversible mitochondrial permeabilization to activation of GSK-3 and subsequent phosphorylation, ubiquitinylation, and degradation of the antiapoptotic BCL-2 family member MCL-1. ...
journal_title:Molecular cell
pub_type: 评论,杂志文章,评审
doi:10.1016/j.molcel.2006.03.005
更新日期:2006-03-17 00:00:00
abstract::Dosage compensation in Drosophila is dependent on MSL proteins and involves hypertranscription of the male X chromosome, which ensures equal X-linked gene expression in both sexes. Here, we report the purification of enzymatically active MSL complexes from Drosophila embryos, Schneider cells, and human HeLa cells. We ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2006.02.007
更新日期:2006-03-17 00:00:00
abstract::X chromosome inactivation (XCI) depends on a noncoding sense-antisense transcript pair, Xist and Tsix. At the onset of XCI, Xist RNA accumulates on one of two Xs, coating and silencing the chromosome in cis. The molecular basis for monoallelic Xist upregulation is not known, though evidence predominantly supports a po...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2006.01.028
更新日期:2006-03-03 00:00:00
abstract::K-Ras associates with the plasma membrane (PM) through farnesylation that functions in conjunction with an adjacent polybasic sequence. We show that phosphorylation by protein kinase C (PKC) of S181 within the polybasic region promotes rapid dissociation of K-Ras from the PM and association with intracellular membrane...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2006.01.012
更新日期:2006-02-17 00:00:00
abstract::Posttranslational modification with small ubiquitin-related modifier (SUMO) has emerged as a central regulatory mechanism of protein function. However, little is known about the regulation of sumoylation itself. It has been reported that it is increased after exposure to various stresses including strong oxidative str...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2005.12.019
更新日期:2006-02-03 00:00:00
abstract::Histone H2AX promotes DNA double-strand break (DSB) repair and immunoglobulin heavy chain (IgH) class switch recombination (CSR) in B-lymphocytes. CSR requires activation-induced cytidine deaminase (AID) and involves joining of DSB intermediates by end joining. We find that AID-dependent IgH locus chromosome breaks oc...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2006.01.005
更新日期:2006-01-20 00:00:00
abstract::DNA replication forks pause in front of lesions on the template, eventually leading to cytotoxic chromosomal rearrangements. The in vivo structure of damaged eukaryotic replication intermediates has been so far elusive. Combining electron microscopy (EM) and two-dimensional (2D) gel electrophoresis, we found that UV-i...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2005.11.015
更新日期:2006-01-06 00:00:00
abstract::Conjugation of ubiquitin (Ub) to a protein substrate targets the substrate for degradation or functional modification, which is tightly controlled by diverse mechanisms including phosphorylation of the substrate. An emerging mechanism involves regulation of the E3 Ub ligase, for example, the JNK-dependent phosphorylat...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2005.11.014
更新日期:2006-01-06 00:00:00
abstract::The protein kinase B-RAF is mutated in approximately 7% of human cancers. Most mutations are activating, but, surprisingly, a small number have reduced kinase activity. However, the latter can still stimulate cellular signaling through the MEK-ERK pathway because they activate the related family member C-RAF. We exami...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2005.10.022
更新日期:2005-12-22 00:00:00
abstract::Although DNA looping between the initiator binding sites (iterons) of the replication origin (ori) of a plasmid and the iterons located in a cis-acting control sequence called inc has been postulated to promote negative control of plasmid DNA replication, not only was definitive evidence for such looping lacking, but ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2005.10.037
更新日期:2005-12-22 00:00:00
abstract::Two new studies provide in vivo ( [this issue of Molecular Cell]) and in vitro ( [this issue of Molecular Cell]) evidence that poleta functions to extend 3' strands exchanged during homologous recombination and raise the issue of how TLS polymerases are selected onto different substrates. ...
journal_title:Molecular cell
pub_type: 评论,杂志文章,评审
doi:10.1016/j.molcel.2005.11.018
更新日期:2005-12-09 00:00:00
abstract::Mismatch repair (MMR) is initiated by MutS family proteins (MSH) that recognize DNA mismatches and recruit downstream repair factors. We used a single-molecule DNA-unzipping assay to probe interactions between S. cerevisiae MSH2-MSH6 and a variety of DNA mismatch substrates. This work revealed a high-specificity bindi...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2005.10.014
更新日期:2005-12-09 00:00:00
abstract::During meiosis, recombination between homologous chromosomes generates crossover (CR) and noncrossover (NCR) products. CRs establish connections between homologs, whereas intermediates leading to NCRs have been proposed to participate in homologous pairing. How these events are differentiated and regulated remains to ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2005.09.021
更新日期:2005-11-23 00:00:00
abstract::Viral initiators perform multiple functions in initiation of DNA replication including ori binding, melting, and unwinding, culminating in the formation of a double hexameric (DH) helicase. We have recapitulated the assembly of the papillomavirus E1 initiator DH helicase, providing the first description of how such a ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2005.09.020
更新日期:2005-11-11 00:00:00
abstract::The terminal step in cytokinesis that severs a cell in two-abscission-is poorly understood. In Developmental Cell, Fabbro et al (2005) identify a centrosome protein whose multiple phosphorylations regulate its movement from centrosome to midbody and completion of abscission. ...
journal_title:Molecular cell
pub_type: 杂志文章,评审
doi:10.1016/j.molcel.2005.10.010
更新日期:2005-10-28 00:00:00
abstract::Membrane penetration of nonenveloped viruses is a poorly understood process. We have investigated early stages of this process by studying the conformational change experienced by polyomavirus (Py) in the lumen of the endoplasmic reticulum (ER), a step that precedes its transport into the cytosol. We show that a PDI-l...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2005.08.034
更新日期:2005-10-28 00:00:00
abstract::MicroRNAs (miRNAs) play a pivotal role in the regulation of genes involved in diverse processes such as development, differentiation, and cellular growth control. Recently, many viral-encoded miRNAs have been discovered, for the most part in viruses transcribed from double-stranded DNA genomes. As with their cellular ...
journal_title:Molecular cell
pub_type: 杂志文章,评审
doi:10.1016/j.molcel.2005.09.012
更新日期:2005-10-07 00:00:00
abstract::The transcriptional activity of many sequence-specific DNA binding proteins is directly regulated by posttranslational covalent modification. Although this form of regulation was first described nearly two decades ago, it remains poorly understood at a mechanistic level. The prototype for a transcription factor contro...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2005.08.013
更新日期:2005-10-07 00:00:00
abstract::ER-associated degradation (ERAD) of glycoproteins depends on dual recognition of protein misfolding and remodeling of the substrate's N-linked glycans. After recognition, substrates are retrotranslocated to the cytosol for proteasomal degradation. To explore the directionality of this process, we fused a highly stable...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2005.07.027
更新日期:2005-09-16 00:00:00
abstract::Streptolydigin (Stl) is a potent inhibitor of bacterial RNA polymerases (RNAPs). The 2.4 A resolution structure of the Thermus thermophilus RNAP-Stl complex showed that, in full agreement with the available genetic data, the inhibitor binding site is located 20 A away from the RNAP active site and encompasses the brid...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2005.07.020
更新日期:2005-09-02 00:00:00
abstract::Androgen receptor (AR) plays a critical role in the development and progression of prostate cancer, where it is a key therapeutic target. Here we report that, in contrast to estrogen receptor transcription complexes which form within minutes and recycle hourly, the levels of regulatory regions bound by AR complexes ri...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2005.07.018
更新日期:2005-09-02 00:00:00
abstract::Plus-end tracking proteins, such as EB1 and the dynein/dynactin complex, regulate microtubule dynamics. These proteins are thought to stabilize microtubules by forming a plus-end complex at microtubule growing ends with ill-defined mechanisms. Here we report the crystal structure of two plus-end complex components, th...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2005.06.034
更新日期:2005-08-19 00:00:00
abstract::The identification of metazoan origins of DNA replication has so far been hampered by the lack of a suitable genetic screening and by the cumbersomeness of the currently available mapping procedures. Here we describe the construction of a library of nascent DNA, representative of all cellular origin sequences, and its...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2005.07.005
更新日期:2005-08-19 00:00:00
abstract::Certain nascent peptide sequences, when within the ribosomal exit tunnel, can inhibit translation termination and/or peptide elongation. The 24 residue leader peptidyl-tRNA of the tna operon of E. coli, TnaC-tRNA(Pro), in the presence of excess tryptophan, resists cleavage at the tnaC stop codon. TnaC residue Trp12 is...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2005.06.013
更新日期:2005-08-05 00:00:00
abstract::For many polarized cells, it is critical that the mitotic spindle becomes positioned relative to the polarity axis. This is especially important in yeast, where the site of cytokinesis is predetermined. The spindle position checkpoint (SPOC) therefore delays mitotic exit of cells with a mispositioned spindle. One comp...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2005.05.030
更新日期:2005-07-22 00:00:00
abstract::Apoptosis, a phenomenon that allows the regulated destruction and disposal of damaged or unwanted cells, is common to many cellular processes in multicellular organisms. In humans more than 200 proteins are involved in apoptosis, many of which are dysregulated or defective in human diseases including cancer. A large n...
journal_title:Molecular cell
pub_type: 杂志文章,评审
doi:10.1016/j.molcel.2005.05.026
更新日期:2005-07-01 00:00:00
abstract::Posttranslational modification of proliferating cell nuclear antigen (PCNA), an essential processivity clamp for DNA polymerases, by ubiquitin and SUMO contributes to the coordination of DNA replication, damage tolerance, and mutagenesis. Whereas ubiquitination in response to DNA damage promotes the bypass of replicat...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2005.06.001
更新日期:2005-07-01 00:00:00
abstract::In solid tissues of vertebrates, initiation of mitochondrial DNA replication encompasses a broad zone downstream of the major noncoding region (NCR). In contrast, analysis with two-dimensional agarose gel electrophoresis of mitochondrial DNA replication intermediates in cultured human cells revealed initiation concent...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2005.05.002
更新日期:2005-06-10 00:00:00
abstract::The anaphase-promoting complex (APC) is a ubiquitin ligase that promotes the degradation of cell-cycle regulators. Cdh1p is an APC coactivator that directly binds APC substrates. A genetic screen in budding yeast identified residues within Cdh1p critical for its function. Cdh1p proteins containing mutations within the...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2005.04.022
更新日期:2005-05-27 00:00:00
abstract::Downstream core promoter elements are an expanding class of regulatory sequences that add considerable diversity to the promoter architecture of RNA polymerase II-transcribed genes. We set out to determine the factors necessary for downstream promoter element (DPE)-dependent transcription and find that, against expect...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2005.04.005
更新日期:2005-05-13 00:00:00
abstract::Diaphanous-related formins (DRFs) regulate dynamics of unbranched actin filaments during cell contraction and cytokinesis. DRFs are autoinhibited through intramolecular binding of a Diaphanous autoinhibitory domain (DAD) to a conserved N-terminal regulatory element. Autoinhibition is relieved through binding of the GT...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2005.04.002
更新日期:2005-04-29 00:00:00
abstract::Comparisons of bacteriophage PRD1 and adenovirus protein structures and virion architectures have been instrumental in unraveling an evolutionary relationship and have led to a proposal of a phylogeny-based virus classification. The structure of the PRD1 spike protein P5 provides further insight into the evolution of ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2005.03.019
更新日期:2005-04-15 00:00:00
abstract::The IkappaB kinases IKKalpha and IKKbeta regulate distinct cytoplasmic and nuclear events that are critical for cytokine-mediated activation of the NF-kappaB pathway. Because the IKKs have previously been demonstrated to associate with the nuclear hormone receptor coactivator AIB1/SRC-3, the question of whether either...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2005.03.006
更新日期:2005-04-01 00:00:00
abstract::The Mediator complex connects transcriptional activators bound at enhancers with RNA polymerase (Pol) II. demonstrate that Mediator also has an important role in transcriptional activation after recruitment of the Pol II machinery to promoters. ...
journal_title:Molecular cell
pub_type: 评论,杂志文章,评审
doi:10.1016/j.molcel.2005.03.002
更新日期:2005-03-18 00:00:00
abstract::The activation of Rab GTPases is a critical focal point of membrane trafficking events in eukaryotic cells; however, the cellular mechanisms that spatially and temporally regulate this process are poorly understood. Here, we identify a null allele of ELP1 as a suppressor of a mutant in a Rab guanine nucleotide exchang...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2005.02.018
更新日期:2005-03-18 00:00:00
abstract::In this study, we used Saccharomyces cerevisiae to identify a biological network that prevents the deleterious effects of endogenous reactive oxygen species. The absence of Tsa1, a key peroxiredoxin, caused increased rates of mutations, chromosomal rearrangements, and recombination. Defects in recombinational DNA doub...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2005.02.008
更新日期:2005-03-04 00:00:00
abstract::E1 enzymes activate ubiquitin or related proteins and pass them to E2 enzymes. A recent structure and associated biochemical studies (Huang et al., 2005) show how an E1 binds its cognate E2 and indicates that large, conformational changes will be an integral component of the E1 reaction cycle. ...
journal_title:Molecular cell
pub_type: 杂志文章,评审
doi:10.1016/j.molcel.2005.02.004
更新日期:2005-02-18 00:00:00
abstract::In Saccharomyces cerevisiae, telomere replication occurs in late S phase and is accompanied by dynamic remodeling of its protein components. Here, we show that MRX (Mre11-Rad50-Xrs2), an evolutionarily conserved protein complex involved in DNA double-strand break (DSB) repair, is recruited to the telomeres in late S p...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2005.01.014
更新日期:2005-02-18 00:00:00